Comparison of Quantitative and Qualitative Tests for Glucose-6-Phosphate Dehydrogenase Deficiency
نویسندگان
چکیده
A barrier to eliminating Plasmodium vivax malaria is inadequate treatment of infected patients. 8-Aminoquinoline-based drugs clear the parasite; however, people with glucose-6-phosphate dehydrogenase (G6PD) deficiency are at risk for hemolysis from these drugs. Understanding the performance of G6PD deficiency tests is critical for patient safety. Two quantitative assays and two qualitative tests were evaluated. The comparison of quantitative assays gave a Pearson correlation coefficient of 0.7585 with significant difference in mean G6PD activity, highlighting the need to adhere to a single reference assay. Both qualitative tests had high sensitivity and negative predictive value at a cutoff G6PD value of 40% of normal activity if interpreted conservatively and performed under laboratory conditions. The performance of both tests dropped at a cutoff level of 45%. Cytochemical staining of specimens confirmed that heterozygous females with > 50% G6PD-deficient cells can seem normal by phenotypic tests.
منابع مشابه
Clinical Manifestations of Acute Hemolysis in Children with Glucose-6-Phosphate Dehydrogenase Deficiency in Fars Province, Iran
Background: Absence or deficiency in Glucose-6-phosphate dehydrogenase (G6PD) enzyme in patients with G6PD deficiency presents with a wide spectrum of symptoms. This study evaluates the clinical features of acute hemolysis in children with G6PD deficiency. Materials and Methods: Seventy G6PD deficient children younger than 18 years old were included in this cross sectional study in Dastgheib H...
متن کاملMOLECULAR IDENTIFICATION OF THE MOST PREVALENT MUTATION OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PD) GENE IN DEFICIENT PATIENTS IN GILAN PROVINCE
Glucose-6-Phosphate Dehydrogenase (G6PD) is a cytosolic enzyme which its main function is to produce NADPH in the red blood cells by controlling the step from Glucose-6-Phosphate to 6-Phospho gluconate in the pentose phosphate pathway. G6PD deficiency is the most common X-chromosome linked hereditary enzymopathy in the world, that result in reduced enzyme activity and more than 125 different mu...
متن کاملPrevalence of Glucose-6-Phosphate Dehydrogenase Deficiency among Male Donors in Shiraz, Southern Iran
The overall incidence of glucose-6-phosphate dehydrogenase (G6PD) deficiency in Iranian population is estimated around 10%-14.9%. G6PD deficiency is an X-linked disorder and 80% of donors are usually male. At present, donors’ blood is not routinely screened for G6PD deficiency in Iran blood bank where for detecting such enzyme deficiency, reliance is placed on pre-donation data. Thus, the G6PD ...
متن کاملPossible Association between Glucose-6-Phosphate Dehydrogenase Deficiency and the Development of Preeclampsia
Glucose-6-Phosphate dehydrogenase (G6PD) deficiency is a common enzyme deficiency in the world. It's Prevalence inIranis about 12% in male & about 1% in female. The present study did examine the relation between the development of preeclampsia and G6PD deficiency. It was investigated whether or not the risk of preeclampsia in G6PD deficient women is higher than that in normal pregnant women.A t...
متن کامل